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銀納米粒子用于無溶劑檢測小分子和質荷比校準的激光解吸/電離質譜

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發表于 2019-1-22 14:16:37 | 只看該作者 回帖獎勵 |倒序瀏覽 |閱讀模式
Silver nanoparticles for solvent-free detection of small molecules and mass-to-charge calibration of laser desorption/ionization mass spectrometry
銀納米粒子用于無溶劑檢測小分子和質荷比校準的激光解吸/電離質譜
Journal of Vacuum Science & Technology B 37, 012906 (2019);

https://doi.org/10.1116/1.5050878
Vadym Prysiazhnyi1,a), Filip Dycka1, Jiri Kratochvil1, Vitezslav Stranak1, Petra Ksirova2, and Zdenek Hubicka1
Hide Affiliations
1University of South Bohemia Faculty of Science, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic
2Institute of Physics ASCR, Na Slovance 2, 18221 Prague 8, Czech Republic
a)Author to whom correspondence should be addressed: [email protected]

ABSTRACT
摘要

This work aims at the utilization of nanostructured surfaces for advanced mass spectrometry [laser desorption/ionization mass spectrometry (LDI MS)].。The authors demonstrate that prepared nanostructures enable independent mass-to-charge calibration and also effectively substitute protonation agent for low-mass molecules instead of conventionally used matrices. Silver nanostructured surfaces were formed as homogeneous thin film, isolated nanoislands, and spherical nanoparticles. Besides the surface characterization, the paper focuses on the impact of LDI MS laser, irradiating the nanostructured surfaces, which results in the production of charged Ag clusters. Irradiated nanoparticle-based surfaces mostly provide single ionized species Ag+ while positive (Agn+, n ≤ 5) and negative ions (Agn−, n ≤ 7) were observed from the nanoislands film. It is shown that the ratio between particular ion line intensities can be tailored by the deposition time. The pattern of silver ions Agn (due to two natural isotopes) can be used for mass-to-charge calibration up to 1000 m/z. Additionally, the silver protonation improves the identification of small molecules. It is demonstrated on detection of sucrose (342.3 g/mol), fructose (180.2 g/mol), and creatinine (113.1 g/mol) molecules.

        這項工作旨在利用納米結構表面進行高級質譜分析[激光解吸/電離質譜(LDI MS)]。 作者證明,制備的納米結構能夠實現獨立的質荷比校準,并且有效地替代用于低質量分子的質子化試劑,而不用常規的材料。形成了的銀納米結構表面為均勻薄膜、分離的納米島和球形納米顆粒。除了表面表征外,本文還重點研究了LDI MS激光照射納米結構表面的影響,由照射產生了帶電的Ag團簇。受輻照的基于納米顆粒的表面主要提供單電離物質Ag+,而從納米島膜層上可觀察到正離子(Agn+, n ≤ 5)和負離子(Agn−, n ≤ 7)。這一結果表明,特定的離子線強度之間的比例可以通過沉積時間來調整。銀離子Agn的模式(由于兩種天然同位素)可用于高達1000 m/z的質荷比校準。另外,銀質子化改善了小分子的識別水平。通過檢測對蔗糖(342.3 g/mol),果糖(180.2 g/mol)和肌酸酐(113.1 g/mol)分子的檢測證明了這一點。



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